paratuberculosis

• MicroRNA dysregulation in cow's jejunum, jejunal lymph node, and caecal Peyer's patch during Mycobacterium avium subsp. paratuberculosis infection
  Mycobacterium avium subsp. paratuberculosis (MAP) is responsible for Johne's disease (JD), a contagious granulomatous gastroenteritis with global distribution. This study investigated the expression of miRNAs and their potential regulatory mechanisms in Canadian Holstein cows with different MAP infection status, including 4 infected (MAPI), 5 tolerant to MAP infection (MAPT) and 5 healthy controls (HC).Intestinal tissues, […]
• Association of Mycobacterium Avium paratuberculosis with Crohn's Disease: A large multicentre study from TB endemic region
  BACKGROUND: The causal relationship between Crohn's disease (CD) and Mycobacterium avium subspecies paratuberculosis (MAP) remains controversial.
• A bayesian analysis of phage-based qPCR and liquid culture for the early detection of Mycobacterium avium subsp. paratuberculosis in young dairy calves
  Bovine paratuberculosis (PTB), caused by Mycobacterium avium subsp. paratuberculosis (MAP), is an endemic infectious disease causing significant economic losses, with infection typically occurring in the first months of life. However, early diagnostic in subclinical infected calves is challenging due to the paucibacillary nature and intermittent shedding of the pathogen, which limits the sensitivity (Se) of […]
• Johne's Disease Control in Beef Cattle: Balancing Test-and-Cull Strategies with Economic and Epidemiological Trade-Offs
  Johne's disease (JD) is a chronic infection of cattle that undermines herd productivity and profitability. While test-and-cull programs are commonly proposed for control, their effectiveness and economic feasibility remain uncertain in beef production systems. This study used an updated agent-based model (ABM) to simulate JD transmission in a representative 300-cow Western Canadian beef herd, coupled […]
• Evaluation of the reproducibility and performance characteristics of the Phagomagnetic separation-qPCR assay for rapidly detecting viable Mycobacterium avium subsp. paratuberculosis in bovine milk and feces
  Inter-laboratory trials were carried out to evaluate the reproducibility and estimate test performance characteristics of the Phagomagnetic separation (PhMS)-qPCR assay, a novel phage-based assay recently developed as a rapid alternative to culture for detecting viable Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk and feces. Unique reagents and a detailed instruction manual required for the […]
• Cytokeratin expression and distribution pattern of epithelioid macrophages in animals with different pathological forms of bovine paratuberculosis: potential role in resilience to PTB
  Paratuberculosis (PTB) is a chronic enteritis caused by Mycobacterium avium subsp. paratuberculosis (Map). Genome-wide association studies revealed a significant enrichment of the keratinization pathway in cattle with multifocal lesions, suggesting a potential role of cytokeratins (CKs) in PTB resilience mechanisms. To confirm this, the amount of CK-expressing cells was analyzed in granulomas from the distal […]
• Systematic evaluation and optimization of TaqMan qPCR assays targeting F57, ISMAP02, and IS900 for multiplex detection of Mycobacterium avium subsp. paratuberculosis
  This study aimed to develop a multiplex quantitative PCR (qPCR) assay for the detection of Mycobacterium avium subsp. paratuberculosis (MAP), the etiological agent of paratuberculosis disease, a chronic and endemic infectious disease affecting ruminant livestock worldwide. Infected animals may remain asymptomatic for years while intermittently shedding MAP into their environment through feces, contributing to ongoing […]
• A dataset on the phylogeny of Mycobacterium avium subsp. paratuberculosis isolated from patients in a referral gastrointestinal diseases centre in the Sudan
  Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis in animals, a disease characterized by chronic intestinal inflammation. MAP has also been detected in patients with Crohn's disease and other illnesses, raising concerns about its potential public health implications. Recently, we isolated MAP from tissue and stool samples of human patients with gastrointestinal […]
• Can the Grana Padano and Parmigiano Reggiano production process guarantee a reduction in pathogenic microorganisms equivalent to the pasteurization process?
  Italian hard cheeses made in the Pianura Padana area, such as Grana Padano and Parmigiano Reggiano, are traditionally produced from raw milk and undergo extended ripening periods. These processes generate multiple microbiological hurdles that can inactivate pathogens. However, current European regulations do not formally recognize the impact of these hurdles as equivalent to pasteurization, limiting […]
• Metabolomic signatures of tuberculosis and paratuberculosis in goats revealed by high-resolution and benchtop NMR spectroscopy
  Goats represent a significant reservoir for tuberculosis (TB) in animals, contributing notably to public and animal health challenges, causing economic repercussions. Ante mortem diagnosis of TB is hindered by the limited sensitivity of available techniques and false-positive results from other mycobacterial infections, such as paratuberculosis (PTB). Nuclear Magnetic Resonance (NMR)-based metabolomics provides unique fingerprinting of […]
• Short Chain Fatty Acids Lower Inflammation and Restore Intestinal Integrity and Function Markers in Mycobacterium paratuberculosis-Infection In Vitro Model
  Background: Infection with Mycobacterium avium paratuberculosis (MAP) is closely associated with Crohn's disease (CD) development, where excessive inflammation and marked intestinal damage are observed. Objectives: In this study, the role of short chain fatty acids, including propionic acid (PPA) and butyric acid (BA), was evaluated in an in vitro model, mimicking CD characteristics. Methods: MAP-infected […]
• Mycobacteriophage Mcgavigan Uses Noncanonical Bxb1-Like Repressor for Heterotypic Superinfection Immunity
  Mycobacteriophage Mcgavigan could be a promising candidate for use as a preventative agent against infections with Mycobacterium avium subsp. paratuberculosis. Bioinformatic analysis of the Mcgavigan genome revealed the presence of an operon containing a "Bxb1-like" repressor. The operon may have been acquired by the phage through horizontal gene transfer with a Bxb1-like mycobacteriophage in its […]
• MicroRNA-mediated regulatory mechanisms in cow's ileum and ileum lymph node in response to Mycobacterium avium subspecies paratuberculosis infection
  Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease (JD), a chronic enteritis infection affecting ruminants with significant economic implications for the dairy industry. The molecular regulatory mechanisms governing host responses to MAP infection remain unclear. This study elucidated the roles of microRNAs (miRNAs) in the ileum (IL), ileal lymph node (ILLN), and liver of cows […]
• Evidence of mycobacteriaemias and mycobacterial co-infections uncovered in cattle at slaughter using a novel phage-based PhMS-qPCR assay for viable Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis
  Accurate diagnosis of Bovine Tuberculosis (bTB) in cattle is important for success of eradication programmes, but the current reliance on the single intradermal comparative cervical tuberculin (SICCT) test is not achieving disease eradication in all jurisdictions, including Northern Ireland (NI). In this study, a novel Phagomagnetic separation (PhMS)-qPCR assay to rapidly detect viable Mycobacterium bovis […]
• Association of SLC11A1 3'UTR (GT)(n) Microsatellite Polymorphisms with Resistance to Paratuberculosis in Sheep
  Paratuberculosis (Johne's disease) is a chronic enteric infection of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP), leading to significant economic losses in livestock production. While the solute carrier family 11 member 1 (SLC11A1) gene has been implicated in resistance to intracellular pathogens in several species, its role in ovine paratuberculosis remains largely uncharacterized. The […]
• Mycobacterium avium subspecies paratuberculosis (MAP) shedding and seropositivity in youngstock in MAP-infected dairy herds in Alberta, Canada
  Johne's disease (JD) control and eradication programs aim to reduce the prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infections and associated economic losses. Youngstock are particularly vulnerable to MAP infection-they can shed MAP in feces, develop antibody titers, and transmit the pathogen to pen mates. However, most JD control programs do not include youngstock in […]
• Integrating transcriptomic and genomic studies for the identification of expression quantitative trait loci associated with bovine paratuberculosis
  The study of the genetic basis underlying the host response to Mycobacterium avium subsp. paratuberculosis (MAP) is usually performed using genome-wide association studies (GWAS), which assess the individual association between genotyped markers, typically single-nucleotide polymorphisms (SNPs), and phenotypic traits of interest (quantitative or qualitative). However, most SNPs identified through GWAS are located in non-coding regions, […]
• Genomic insights and functional adaptation of <em>Mycobacterium intracellulare</em> strains isolated from patients with inflammatory bowel disease
  In clinical practice, inappropriate diagnosis of inflammatory bowel disease (IBD) ascribing the disease to Mycobacterium avium paratuberculosis (MAP) and resulting mistreatment has been a challenge for healthcare workers. This highlights the need to accurately identify and characterize MAP members in patients with gastrointestinal (GI) ulceration. A total of 889 patients exhibiting symptoms of IBD were […]
• Diagnostic and economic evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with machine learning for screening of Johne's disease from dairy cow serum
  Johne's disease (JD) is an infectious bacterial disease (Mycobacterium avium ssp. paratuberculosis) that primarily affects the intestinal tract and associated lymph nodes of ruminants, especially in cattle. It is both a herd-level problem and an individual animal problem, as much of the infection is subclinical. This disease has a significant impact due to drastic production […]
• Detection of tissue colonizing bacteria and their association with clinicopathological and molecular features of inflammatory bowel disease
  CONCLUSIONS: These findings suggest that, compared with UC, certain specific gut bacteria, such as Enterotoxigenic Bacteroides fragilis, may play a more important role in the occurrence, development and prognosis of CD. In addition, different intestinal microbiota-based therapeutic strategies should be developed for CD and UC.